Journal: medRxiv

Article Title: Exploratory analyses of Immunologic Features in a Randomized, Placebo-Controlled Trial of Nirmatrelvir/Ritonavir for Long COVID

doi: 10.64898/2026.02.24.26347001

Figure Lengend Snippet: Log-transformed circulating total antibody subtypes and IgG isotypes, as measured by Luminex assays at baseline and day 28 in the two treatment arms. SARS- CoV-2 antibody responses were assessed using C. Nucleocapsid by COBAS assay, ELISA for D . Spike, E . Receptor Binding Domain (RBD), F . Nucleocapsid. Significance for difference in group median values was assessed using Wilcoxon matched-pairs signed-rank test with Benjamini–Hochberg false-discovery rate (FDR) correction for multiple comparisons. PIWAS line profiles of IgG binding within participants along the SARS-CoV-2 G. Spike and H. Nucleocapsid amino acid sequences. 95th percentile values of binding are arranged by group. P-values are provided for statistically significant differences.

Article Snippet: To summarize, 96-well MaxiSorp plates (Thermo Fisher Scientific, #442404) were coated at a concentration of 2 μg/ml in PBS with recombinant SARS-CoV-2 S protein (50 μl per well; ACROBiosystems, #SPN-C52H9-100 μg) or RBD (ACROBiosystems, #SPD-C52H3-100 μg) or nucleocapsid protein (NUN-C5227-100 μg, ACROBiosystems) and incubated overnight at 4°C.

Techniques: Transformation Assay, Luminex, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: ACS Nano

Article Title: Utilizing Constrained Bicyclic Peptides for In Vitro Diagnostics

doi: 10.1021/acsnano.5c19041

Figure Lengend Snippet: (A) Biorecognition pair ELISA signal-to-noise matrix heatmap at 50 ng·mL –1 SARS-CoV-2 N protein, n = 3. (B) ELISA calibration curves of the best performing pairs for the detection of the SARS-CoV-2 N protein, data shown as mean ± S.D, n = 3. (C) ELISA cross-reactivity study for 1 μg·mL –1 of HCoV N proteins, data shown as mean ± S.D, n = 3. (D) Biorecognition pair NLISA signal-to-noise matrix heatmap at 10 ng·mL –1 SARS-CoV-2 N protein, n = 3. (E) NLISA calibration curves of the best performing pairs for the detection of the SARS-CoV-2 N protein, data shown as mean ± S.D, n = 3. (F) NLISA cross-reactivity study for 1 μg·mL –1 of HCoV N proteins, data shown as mean ± S.D, n = 3. L D refers to the decision limit, which is calculated as the mean of the blank plus three times the standard deviation of the blanks ( S 0 + 3σ S 0 ), whilst LoD refers to the limit of detection as calculated via a statistical method derived from Holstein et al., which considers the variance of the blank samples and test samples.

Article Snippet: The plate was then washed (3×) with PBST (400 μL·well –1 ) and 100 μL recombinant SARS-CoV-2 nucleocapsid protein (Sino Biological; cat: 40588-V08B) in PBST at 50 ng·mL –1 was added and incubated at room temperature for 30 min with PBST as a negative control.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation, Derivative Assay

Journal: ACS Nano

Article Title: Utilizing Constrained Bicyclic Peptides for In Vitro Diagnostics

doi: 10.1021/acsnano.5c19041

Figure Lengend Snippet: (A) Strips showcasing biorecognition element pair LFIA signal-minus-noise matrix at 1 ng·mL –1 SARS-CoV-2 N protein. (B) Corresponding biorecognition element pair LFIA signal-minus-noise matrix heatmap at 1 ng·mL –1 SARS-CoV-2 N protein. (C) Representative strips showing preamplification (top) and amplified (bottom) test line intensities at a serial dilution of SARS-CoV-2 N protein. Stars indicate LFIA strip with the lowest antigen concentration that can be seen visually by the naked eye. (D) Corresponding calibration curves showing normalized test line intensities for the preamplified and amplified detection of SARS-CoV-2 N protein in LFIA, data shown as mean ± S.D, n = 3. (E) LFIA cross-reactivity study for 1 μg·mL –1 of HCoV N proteins.

Article Snippet: The plate was then washed (3×) with PBST (400 μL·well –1 ) and 100 μL recombinant SARS-CoV-2 nucleocapsid protein (Sino Biological; cat: 40588-V08B) in PBST at 50 ng·mL –1 was added and incubated at room temperature for 30 min with PBST as a negative control.

Techniques: Amplification, Serial Dilution, Stripping Membranes, Concentration Assay

Journal: ACS Nano

Article Title: Utilizing Constrained Bicyclic Peptides for In Vitro Diagnostics

doi: 10.1021/acsnano.5c19041

Figure Lengend Snippet: (A) Representative LFIA showing test line intensities at a serial dilution of SARS-CoV-2 N protein spiked in pooled saliva/running buffer matrix utilizing AuNPs. Star indicates LFIA strip with the lowest antigen concentration that can be seen visually by the naked eye. (B) LFIA cross-reactivity study for 1 μg·mL –1 of HCoV N proteins.

Article Snippet: The plate was then washed (3×) with PBST (400 μL·well –1 ) and 100 μL recombinant SARS-CoV-2 nucleocapsid protein (Sino Biological; cat: 40588-V08B) in PBST at 50 ng·mL –1 was added and incubated at room temperature for 30 min with PBST as a negative control.

Techniques: Serial Dilution, Stripping Membranes, Concentration Assay